Genetic diversity of rose germplasm in Pakistan characterized by random amplified polymorphic DNA (RAPD) markers

摘要

Random amplified polymorphic DNA (RAPD) markers have been found to be very useful in studying the genetic variability of different species, including?Rosa. Present studies were undertaken for the identification and analysis of genetic variation within a collection of 4 species and 30 accessions of rose using RAPD analysis technique. The results showed the molecular distinctions among the genotypes when analysed using 25 RAPD primers.?Total amplified bands were 146, out of which 110 werepolymorphic, with an average of seven bands per primer.?Maximum number of bands (10) was produced by primer GLD-20, while GLC-02 produced the minimum number of bands (2). Maximum polymorphism in the present study was obtained by primer GLA-03, GLA-05, GLA-07, GLA-10, GLC-02, GLC-06, GLC-08, GLC-10, OPG-11 and OPE-19 which produced 100% polymorphic bands, while primer GLB-11 produced only 42.85% polymorphic bands. This study demonstrated the potential of RAPD technique for the characterization of genetic variation within the rose germplasm.