Identification of single nucleotide polymorphism of growth hormone gene exon 4 and intron 4 in Pesisir cattle, local cattle breeds in West Sumatera Province of Indonesia

摘要

The pupose of this study?was?to identify genetic polymorphisms of bovine?growth hormone gene exon?4, and intron?4?in local cattle breeds in West Sumatera Province of Indonesia.?DNA?was?isolated from 60 blood samples and polymerase chain reaction (PCR) product of GH5?fragment (366?bp) were?directly?sequenced.?Multiple alignments, including 60 bGH DNA sequences obtained by direct sequencing and bGH sequences from a public database (National Center for Biotechnology Information, acces number?M57764), revealed 15 polymorphisms (five?SNP,?eightdeletion,?and two?insertion). Eight?deletions were detected in position?1740, 1743,1745,?1747, 1749, 1750, 1753, and 1754?with frequency allele of?0.50,?0.22,?0.125,0.53, 0.06,?0.06?and?0.83, respectively. Besides,?two insertions?C?were detected in position 1790 and?1895?with frequency allele of 0.06 and 1.00,?respectively.?Fivemutations were detected in position 1914, 1930, 1947, 1980, and 2025?with genotypesC?à?G, G?à?A,?T?à?G, T?à?C, and?A?à?G respectively with frequency allele of 0.40, 0.48,?1.00,?0.42, and 0.38, respectively.?Another deletion at position 1740, 1743, and 1754 changed?codon?CAG, TCG, TGG, CTT, GGG, CCC?to?codon CGT, GTG, GCT, TGG, GCC?and new deletion at position?1745, 1749,?and?1754?changed?the codonTCG, TGG, CTT, GGG, CCC, CTG?to?TCG, GGC, TGG, GCC, CTG.?Deletion atposition?1749?and?1754?changed?the codon?TCG, TGG, CTT, GGG, CCC,?and?CTG?to TCG,?TGG,?CTG,?GGC,?CCT.?These data provide evidence that?GH?gene?of this breed is slightly different from other breeds. This?polymorphic source can be used?to refer to?performance and?to?investigate whether these polymorphics?are?responsible for quantitative variation in growth.