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首页> 外文期刊>African Journal of Biotechnology >Comparison of broiler performance, carcass yields and intestinal microflora when fed diets containing transgenic (Mon-40-3-2) and conventional soybean meal
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Comparison of broiler performance, carcass yields and intestinal microflora when fed diets containing transgenic (Mon-40-3-2) and conventional soybean meal

机译:当饲喂含转基因(Mon-40-3-2)和常规豆粕的日粮时,肉鸡的性能,car体产量和肠道菌群的比较

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This study was conducted to analyze the effects of transgenic glyphosate-tolerant soybeans on the performance, carcass yields and intestinal microflora of broiler chickens. Three hundred and sixty one-day-old Abor Aerec broilers were randomly divided into two dietary treatments, adding?genetically modified?(GM)?glyphosate-tolerant soybean meal?or conventional soybean meal,?respectively. Broiler body weight and feed intake were recorded at regular intervals (day 0, 21 and 42). Chickens were slaughtered at day 42 for carcass yield measurement and sampling. Diversity of the ileum and cecum microflora was determined by denaturing gradient gel electrophoresis (DGGE) technique and DNA sequencing. No treatment differences (P > 0.05) were detected among dietary treatments for any measured performance and carcass parameters. The microbial population in ileum and cecum also had no significant difference between the two treatments (P>0.05). The similarity of the total ileum and cecum microflora between the two treatments was about 62 and 58%, respectively. The DNA-DGGE electrophoresis pattern bands of intestine microbe were divided into two groups because of the different diet. Fifteen DGGE DNA bands were identified, of which five of them were identified as known bacteria. The current study showed that there were no adverse effects of the transgenic soybean meal on the intestinal microflora of broilers.
机译:本研究旨在分析耐草甘膦转基因大豆对肉鸡生产性能,car体产量和肠道菌群的影响。将361只1日龄的Abor Aerec肉鸡随机分为两种饮食处理,分别添加“转基因”(GM)“耐草甘膦豆粕”或常规豆粕。定期记录肉鸡体重和采食量(第0、21和42天)。在第42天将鸡宰杀以进行car体产量测量和取样。回肠和盲肠菌群的多样性通过变性梯度凝胶电泳(DGGE)技术和DNA测序来确定。在饮食治疗中,对于任何测得的性能和car体参数,均未发现治疗差异(P> 0.05)。回肠和盲肠中的微生物种群在两种处理之间也没有显着差异(P> 0.05)。两种处理之间的总回肠和盲肠菌群相似度分别约为62%和58%。由于饮食的不同,肠道微生物的DNA-DGGE电泳图谱带分为两组。鉴定出15条DGGE DNA条带,其中5条被鉴定为已知细菌。目前的研究表明,转基因豆粕对肉鸡肠道菌群没有不利影响。

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