Inhibition of bacterial adhesion to HT-29 cells by lipoteichoic acid extracted from Clostridium butyricum

摘要

The?aim?of?this experiment was to study?the?effect?of?the lipoteichoic acid (LTA) extracted from?Clostridium butyricum?on the adhesion of?C. butyricum?andEscherichia coli?to HT-29 human intestinal cells. The method of extraction of lipoteichoic acid form?C. butyricum?by TX114 was evaluated. The purification of the LTA by DEAE-cellulose 52 anion exchange chromatography was also investigated. In addition, the LTA was assayed for its inhibition of the adhesion of?C. butyricumand?E. coli?to HT-29 cells and antimicrobial activity. Our results showed that LTA could be extracted by TX114 and purified by DEAE-cellulose 52 anion exchange chromatography and could inhibit the adhesion of?C. butyricum?and?E. coli?to HT-29 cells. This result also revealed that the LTA from?C. butyricum?could inhibit the adhesion of?C. butyricum?and?E. coli?to intestinal cells.