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Genetic diversity of sweet potatoes collection from Northeastern Brazil

机译:巴西东北部甘薯品种的遗传多样性

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The sweet potato, Ipomoea batatas (L.) Lam has its origin in Tropical America. In Sergipe State (Brazil), its production is very important, and to explore its potential in local agriculture in the State, the Embrapa Coastal Tableland created a collection with 52 accessions located in Umbaúba City. Some accessions were from germplasm belonging to Embrapa vegetables and others from local farmers of Sergipe. Here, we provide the first data on the genetic diversity and structure of sweet potato collection of SPGB using random amplified polymorphic DNA (RAPD) markers. RAPD data were used to determine genetic variability via a model-based Bayesian procedure (structure) and molecular variance analysis (AMOVA). In addition, Shannon index, genetic diversity and Jaccard coefficients were also estimated. ?RAPD was efficient for the analysis of genetic diversity to identify groups and measure the genetic distance between accessions. The markers showed that the collection had a high level of polymorphism. By UPGMA, we separated three groups of genotypes and identified two reconstructed populations by structure software.
机译:甘薯番薯(Ipomoea batatas(L.)Lam)起源于热带美洲。在Sergipe州(巴西),其生产非常重要,为了发掘该州在当地农业中的潜力,Embrapa沿海高原地区在翁巴乌巴市创建了52个种质的藏品。一些种质来自Embrapa蔬菜的种质,其他种质则来自塞尔希培州的当地农民。在这里,我们提供了使用随机扩增多态性DNA(RAPD)标记对甘薯SPGB的遗传多样性和结构的第一份数据。 RAPD数据用于通过基于模型的贝叶斯方法(结构)和分子变异分析(AMOVA)确定遗传变异性。此外,还估计了香农指数,遗传多样性和雅克卡系数。 RAPD能有效地分析遗传多样性,以鉴定群体并测量种质之间的遗传距离。标记表明该集合具有高水平的多态性。通过UPGMA,我们分离了三组基因型,并通过结构软件鉴定了两个重建的种群。

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