Callus induction and adventitious shoot regeneration in Zizyphus jujuba Mill. Huizao

摘要

This study was conducted to develop a protocol for rapid callus induction and subsequent shoot regeneration in?Zizyphus jujuba?Mill. ‘Huizao’. The best callus induction (95%) was obtained from young expanding leaves between the third and fifth nodes?in vitro?jujube shoots in?MS media supplemented with 0.5 mg/L?benzylamino purine?(6-BA), 2.0 mg/L?2,4-dichlorophenoxyacetic acid? (2, 4-D), and 0.5 mg/L silver nitrate (AgNO3).?Incubation in darkness had non-significant effects on callus induction frequency of leaf explants, while callus formation of leaf was observed five to seven days earlier in one or two weeks of dark incubation. For shoot regeneration, the results showed that combination of zeatin (ZT) and,?indole-3-acetic acid?(IAA) was found to be more effective than that of 6-BA and?indole-3-butyric acid?(IBA). A maximum of?37.34% callus differentiation occurred in MS medium supplemented with2.0 mg/L ZT and 0.1 mg/L IAA.