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首页> 外文期刊>Acta Scientiarum. Agronomy >A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.)
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A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.)

机译:RAPD和ISSR标记的比较揭示了甘薯地方品种(Ipomoea batatas(L.)Lam。)之间的遗传多样性。

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摘要

Small farmers have played an important role in maintaining genetic resources of traditional varieties. Collecting and characterizing germplasm, especially landraces and heirloom varieties, are strategic activities that would create a knowledge base describing the traditional varieties of a given region and reduce the risk of genetic erosion. The objectives of this work were as follows: collect sweet potato roots from rural properties and local markets in Rio de Janeiro State, Brazil; characterize the accessions using RAPD and ISSR molecular markers, comparing the efficiency in discrimination of these two types of molecular markers; and estimate the genetic variability of the population. Fifty-nine samples from rural properties and 19 from local markets were studied. A matrix of binary data was developed to analyze the molecular data using the Jaccard index to estimate genetic differences among accessions, and clusters were made using the UPGMA method. The molecular techniques detected genetic variability among the accessions, with cophenetic correlation coefficients (CCC) of 0.80 for RAPDs and 0.89 for ISSRs. The RAPD and ISSR marker results corresponded well, with a correlation of 0.55. All of the accessions were considered distinct, which demonstrates that traditional farmers maintain sweet potato genotypes that have good genetic diversity.
机译:小农户在维护传统品种遗传资源方面发挥了重要作用。收集和定性种质,特别是地方品种和传家宝品种,是一项战略活动,它将建立一个描述给定地区传统品种的知识库,并减少遗传侵蚀的风险。这项工作的目标如下:从巴西里约热内卢州的农村地产和当地市场收集甘薯根;使用RAPD和ISSR分子标记物表征种质,比较两种分子标记物的鉴别效率;并估算种群的遗传变异性。研究人员从农村物业中抽取了59个样本,从本地市场中抽取了19个样本。开发了二进制数据矩阵,使用Jaccard指数分析了分子数据,以评估种质间的遗传差异,并使用UPGMA方法进行了聚类。分子技术检测到这些种质之间的遗传变异性,对于RAPDs,对于ISSRs,其同系相关系数(CCC)为0.80。 RAPD和ISSR标记结果吻合良好,相关系数为0.55。所有的种质都被认为是不同的,这表明传统的农民保持着具有良好遗传多样性的甘薯基因型。

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