Biochemical and molecular investigations on prime mechanisms of induced resistance in French bean (Phaseolus vulgaris L.) and soybean (Glycine max L.)

摘要

The prime mechanisms of induced resistance in French bean and soybean plants were investigated against three pathogenic fungi, namely?Fusarium solani?f. sp.?phaseoli, Fusarium solani?f. sp.?glycine?andColletotricum lindemothianum. This latter fungal pathogen was chosen for a comparative purpose, as it represents a non related genus of the main fungal pathogens used in this study. The efficacy of the induced resistance was tested using four different incubation temperatures (20, 25, 30 and 35°C), in combination, with the use of the non pathogenic?Fusarium, isolated from potatoes, served as an inducer or elicitor for 4 h and, subsequently, the challenged hypocotyls were inoculated with the pathogenic isolate of?F. solani?f. sp.phaseoli?in all the pathosystems tested in this study. Among the tested cultivars, Nebraska was moderately resistant and Crowford was moderately susceptible to?Fusarium?infection, whereas the other tested cultivars were susceptible. randomly amplified polymorphic DNA (RAPD) fingerprinting profiles characterized the tested cultivars and pathogens genomes, whereas SDS protein electrophoresis showed the newly induced proteins as a result of prime mechanisms of induced resistance.