Effect of epigallocatechin-3-gallate on rat adipocyte lipolysis and HSL,TGH transcriptional expression

摘要

To study the lipolytic mechanism of EGCG, effects of EGCG (epigallocatechin-3-gallate) on lipolysis in rat primary adipocytes and mRNA expression of hormone-sensitive lipase (HSL)，triacylglycerol hydrolase (TGH) were investigated through determination of glycerol release，triglyceride (TG) content and RT-PCR. The results showed that, EGCG of lower concentration (5 μmol/L) had no effects on glycerol release of mature adipocytes, while higher concentration could remarkable stimulate glycerol release compared with control group. Meanwhile, TG content in adipocytes of treated groups decreased in dose-dependent manners, proving that EGCG is an effective accelerant to lipolysis. Additionally, EGCG significantly down-regulated TGH and HSL mRNA expression, and the expression of TGH and HSL mRNA were very low when treated with 200 μmol/L EGCG[Acta Zoologica Sinica 53(4): 767–772, 2007].