Phylogenetic Analysis of Bovine Herpesvirus Isolate of India

摘要

Bovine herpesvirus-1 (BHV-1) causes infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis / balanoposthitis (IPV/IPB) in cattle and buffaloes. BHV-1 is closely related to bovine herpesvirus-5 (BHV-5), which has been recovered from both genital and respiratory tracts of cattle and buffaloes. Perusal of literature reveals paucity of information on genetic characteristics of BHV subtypes circulating in India. In the present study, 25 isolates originated from five different states of India viz. Gujarat, Uttar Pradesh, Maharashtra, Karantaka and Andhra Pradesh, during the period 1983-2010, were genetically characterized by restriction endonuclease analysis (REA) and partial sequencing of unique long (UL27, UL44) and unique short regions (US1.67) of the viral genome. The REA patterns (Hind III) of these isolates indicated that they were indistinguishable from BHV-1.1 subtype. The phylogenetic analysis based on nucleotide sequences of the unique long (UL) and unique short (US) regions revealed the high sequence homology (>99%) within the isolates and their close relatedness to the BHV-1.1 subtype. The present study established the prevalence of BHV-1.1 as the predominant circulating subtype of BHV in India. The current study also confirmed that the genomic fingerprinting based on HindIII cleavage, direct sequencing of gC (UL44) and gB (UL27) gene-derived PCR amplicons were useful tools for genetic characterization of BHV strains