Use of Polymeric Scaffold for In Vitro Growth of Fibroblast-Like Cells of Indian Major Carp, Cirrhinus Mrigala

摘要

Cell culture has many applications in biological research. Now days, the focus has been to replace routinely practiced two–dimensional (2–D) cell culture with three–dimensional (3–D) cell culture systems due to their efficacy in increasing cell attachment, proliferation and differentiation. In this study, formulated poly lactic acid (PLA) scaffold blended with different natural polymers such as gelatin (PLA–G), alginate (PLA–A), dextran (PLA–D) and pectin (PLA–P), by multiple emulsion solvent evaporation method, were evaluated to find their effect on the attachment, growth and proliferation of fibroblast–like cells from heart of Indian Major Carp, Cirrhinus mrigala. Amongst the formulated scaffolds, PLA–A demonstrated significantly better growth of fibroblast–like cells as compared to control and other formulations. The effect of different seeding density (0.4, 0.6, 0.8, 1.2 and 1.6 × 10 ⁵ cells ml^–1 media) on cell proliferation and growth was also studied using PLA–A. PLA–A demonstrated more than 1.5 times higher cell proliferation and growth as compared to control (2–D) cell culture after 9th day post seeding, even with less cell seeding density (1.6 × 10⁵ cells ml^–1 media). However, with a seeding density of 0.8 × 10⁵ cells ml ^–1 media, this scaffold formulation (PLA–A) supported best proliferation and growth of fibroblast cells. In short, PLA–A based hydrophilic scaffold formulation revealed its potential use in culture of anchorage dependent fibroblast–like cells from heart of Indian major carp, C. mrigala.