MALE-SPECIFIC PROTEIN OF INDIAN MAJOR CARP, LABEO ROHITA (HAM.)-A KEY FOR SEX DIFFERENTIATION AND BROOD STOCK MANAGEMENT

摘要

In non-breeding seasons brood stock management is very much essential to ensure the stocking of appropriate number of males and females for effective breeding in breeding season. But, in non-breeding seasons, sex differentiation of Indian major carps (L. rohita) is impossible without sacrificing the fish, as no specific morphological or genetic markers are reported. On analysis of serum and mucus samples from all seasons, a monomelic glycoprotein of molecular weight 17.85 kDa was detected in male samples only. The male specificity nature of the protein was confirmed through western blotting and immunostaining. Thus, in the process we have discovered a protein which is specific to male Indian major caips (L. rohita) and present in all seasons thereby a key for sex differentiation in non-breeding seasons.