Phylogenetic analysis of Escherichia Coli isolated from mastitis milk

摘要

Pathogenic Escherichia coli, when colonize the bovine mammary glands, results in the cell damage as well as changes in the composition of the milk. A total 50 milk samples of Frieswal (HF x Sahiwal) cow herd maintained at Military Farm, Meerut were collected from mastitis (n= 35) and normal (n=15). Escherichia coli were detected through simplex PCR using published primers of 23s rRNA. All the 35 mastitis milk samples were positive for Escherichia coli, which was further confirmed by sequencing and phylogenetic analysis by using MEGA 5.2 software.The Phylogenetic analysis of our isolate (KF278640) with otherEscherichia coli fall in a clade with (AM158281.1) Escherichia coli 16S rRNAtRNA– Glu and 23S rRNA.