Clinical Study with Investigation of Polymorphism FSHR Gene in Twining Goats in Iraq

摘要

This study included the insemination of 15 fertile local Iraqi goats. The experimental goats were examined by abdominal palpation at 45, 60 and 90 days, and were performed ultrasound to confirm the presence or absence of the pregnancy at 30, 35, 50, and 55 days post-insemination. Ultrasonographic examination was performed using prop 3.5MHz for abdominal examination with special gel for the prop. The study aimed to investigate the polymorphism of FSHR gene, which is responsible of fertility by reaction with the FSH hormone. The results revealed that the twining pregnancy was detected in 10 goats while the other 5 goats have single kids. The polymorphism was identified at three cleavage sites (304 bp, 214 bp, and 90 bp) within the amplification fragment. Three genotypes BB (304 bp) twin birth, AA (214bp/90 bp) single birth, and AB (304 bp/214 bp/90bp) twin birth were detected in local Iraqi goat.