Molecular diagnosis of New Duck disease in India by 16SrRNA gene based PCR

摘要

Riemerella anatipestifer causes New Duck disease or infectious serositis which is an epizootic infectious disease in poultry resulting in serious economic losses, especially in the duck industry through high mortality, reduced growth rate, poor feed conversion, increased condemnations and high treatment costs. Precise and rapid identification is especially important in case of R. anatipestifer, as mistaking it for Pasteurella multocida leads to false alarm of fowl cholera, resulting in delayed diagnosis of the disease. This is the first report on molecular biology based confirmation of the occurrence of R. anatipestifer infection in ducks of India. In the present study, isolation of R. anatipestifer was attempted from suspected field outbreaks of which six isolates were obtained, namely KAL-1, KNB-1, KML-1, KML-2, KML-3, and KTL-1. All the isolates that were culturally and biochemically similar to Riemerella spp were subjected to specific 16S rRNA gene based PCR. Sequencing of the amplicons revealed 99% similarity to R. anatipestifer strain ATCC 11845 ribosomal RNA partial sequence. The results confirm that R. anatipestifer is prevalent among Indian ducks against which proper control and therapeutic strategies need to be developed