The Ability of Fresh Pineapple Juice in Reducing the Cytogenetic Effect of Ifosfamide on the Bone Marrow Cells of Male Albino Mice

摘要

The main aim of this study is to evaluate the potential prophylactic and anti-mutagenic role played by a nutritional agent of natural origin i.e.fresh pineapple juice, against the cytotoxicity and genotoxicity exerted by the widely used anti-neoplastic agent, Ifosfamide, on bonemarrow cells of male white mice, as an in vivo model, through the use of the Micronucleus test, which is one of the short term tests approvedfor such studies. Towards the achievement of such a goal, an experiment was conducted on male mice assigned to 4 groups, namely: TheFirst Group or Control Group(C): This group includes male mice treated solely with a physiological solution for five (5) successive days.The Second Group II or Singly Treated Group (P): This group includes male mice treated solely with Pineapple juice at a dose of 0.40ml/kg/day, administered orally for five successive days. The Third Group III or Singly Treated Group (T1): This group includes male micetreated with Ifosfamide at doses of 30, 60 and 150 mg/kg/day, administered by intra-peritoneal injection for five successive days. The FourthGroup IV or Doubly Treated Group (P +T1): This Group includes male mice treated with pineapple juice at a dose of 0.40 ml/kg/day,administered orally, followed by intra-peritoneal injection of the drug (Ifosfamide) at doses of 30, 60 and 150 mg/kg/day for five successivedays. The results of the current study demonstrate the importance of using short term tests to gauge the prophylactic (anti-mutagenic) roleplayed by a certain substance against the genotoxic and cytotoxic effect of chemical agents, including anti-neoplastic agents. It furtherdemonstrated that the ingestion of fresh pineapple juice prior to treatment with ifosfamide had a positive impact against the cytotoxic andgenotoxic effects on subject bone marrow cells, resulting from treatment with Ifosfamide.