Mitochondrial Cytochrome oxidase-I gene molecular marker used to perform the phylogenetic analysis of Aedes aegypti populations collected in western regions of Saudi Arabia

摘要

Mosquitoes of genus Aedes (Stegomyia) contains large number of species of which Aedesa egypti is the dramatic worldwide distribution. These Aedes species are considered of medically important vector for numerous arboviruses such as Dengue, Zika and Chikungunya viruses, which cause a huge medical problems to humans. To combat such virus diseases, the genus and species of mosquitoes must be first identify. The present investigation has aimed to perform the identification and the phylogenetic analysis of Aedesa egypti specimens collected from different western areas of Saudi Arabia using DNA sequencing assay based on cytochrome oxidase-1 (COX-1) gene. 30 mosquito samples were collected from different infected sites in Jeddah, Makkah and Taif regions. The mitochondrial COX-1 gene amplification and sequencing were carried out using the universal primers LCO1490 and HCO2198. The obtained sequences were phylogenetic analyzed and compared with available sequences in GenBank using the alignment search tool in NCBI-Nucleotide database and CLUSTAL-O (1.2.4) multiple sequence alignment. All mosquito samples were morphologically identified as Aedes (Stegomyia) aegypti. Also, all of our COX-1 gene sequences have high matching result in GenBank with KU495081.1, MF999266.1, KY022527.1, MG242463.1 and KT339669.1 of Aedes aegypti mitochondrial COX-1 gene. Aligned of all nucleotide sequences of the COX-1 gene were illustrated in a single data matrix and create two phylogeny trees. In conclusion, our results indicated that the genetic diversity and phylogenetic analysis of the collected Aedes (Stegomyia) aegypti samples may have important role in the arboviruses vector control and understanding the evolutionary processes of the Aedes cryptic species in western regions of Saudi Arabia.