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Screening of antibacterial activity of raspberry (Rubus idaeus L.) fruit and pomace extracts

机译:覆盆子(Rubus idaeus L.)水果和果渣提取物的抗菌活性的筛选

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Antibacterial activity of fruit and pomace extracts (concentration 50 mg/ml) of two raspberry (Rubus idaeus L.) cultivars (Meeker and Willamette) was tested against selected Gram-positive and Gram-negative bacteria (reference and wild strains). Disc diffusion method with 15 μl of extracts and agar-well diffusion method with 50 and 100 μl were used. Antibiotic (cefotaxime/clavulanic acid) was used as a control. Both raspberry fruit extracts showed the strongest antibacterial activity against Pseudomonas aeruginosa (wild strain) and Bacillus cereus, where the largest clear zones (without growth) appeared. Escherichia coli was the most resistant strain, with only zone of reduced growth. The highest antibacterial activity of pomace extracts was against Staphylococcus aureus and Staphylococcus saprophyticus. There were no differences in the antibacterial activity between cultivars for both fruit and pomace extracts. [Projekat Ministarstva nauke Republike Srbije, br. TR 31044].
机译:测试了两个树莓(Rubus idaeus L.)品种(Meeker和Willamette)的果实和果渣提取物(浓度50 mg / ml)对所选革兰氏阳性和革兰氏阴性细菌(参考菌株和野生菌株)的抗菌活性。分别使用15μl提取物的碟片扩散法和50和100μl的琼脂井扩散法。抗生素(头孢噻肟/棒酸)用作对照。两种覆盆子果实提取物均对铜绿假单胞菌(野生菌株)和蜡状芽孢杆菌具有最强的抗菌活性,其中出现了最大的透明区(无生长)。大肠杆菌是最有抵抗力的菌株,仅有减少的生长区。果渣提取物的最高抗菌活性是针对金黄色葡萄球菌和腐生葡萄球菌。果实和果渣提取物的品种之间的抗菌活性没有差异。 [Projekat Ministarstva nauke Republike Srbije,br。 TR 31044]。

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