Enhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific

摘要

In order to determine whether the enhanced specific antibody productivity (qMAb) of calcium alginate-entrapped hybridoma is cell line-specific, calcium alginate-entrapped hybridomas (4A2 and DB9G8) were cultivated under the condition where we had previously observed significantly enhancedqMAbof calcium alginate-entrapped S3H5/γ2bA2 hybridoma. Unlike S3H5/γ2bA2 hybridoma, neither 4A2 nor DB9G8 hybridomas showed persistently enhancedqMAbwhen they were entrapped in calcium alginate beads. The enhancedqMAbof entrapped 4A2 and DB9G8 hybridomas, which was 2–3 times higher than theqMAbof free-suspended cells in a control experiment, was observed only during the early stage of the culture. During the early stage of the culture, the viable cell concentration decreased probably due to cell damage during the entrapment process. As cell growth resumed, theqMAbdecreased to the similar level ofqMAbof free-suspended cells within 5–7 days. Thus, we conclude that the enhancedqMAbof calcium alginate-entrapped hybridomas is cell line-specific.