Influence of different iodinated contrast media on the induction of DNA doublea??strand breaks after in vitro Xa??ray irradiation

摘要

The objective of this work was to examine differences in DNA doublea??strand break induction in peripheral blood lymphocytes after in vitro Xa??ray irradiation between iodinated contrast agents. Four different iodinated Xa??ray contrast agents a?? three of them with two different iodine concentrations a?? and mannitol (negative control; concentration of 150a??mg mannitol per ml blood) were pipetted into blood samples so that there was a concentration of 0, 7.5 or 15a??mg of iodine per ml blood in the samples. Negative controls without contrast medium (0a??mg of iodine per ml blood) were also processed for every irradiation dose. The tubes were exposed to 0, 20 or 500a??mGy in vitro Xa??ray irradiation. After that, the lymphocytes were separated by using densitya??gradient centrifugation. Fluorescence microscopy was applied to determine the average number of ?3H2AXa??foci per lymphocyte in the presence or absence of different contrast media or mannitol. Differences in the number of ?3H2AXa??foci were statistically analysed by onea??way ANOVA and posta??hoc Tukey's honestly significant difference test. Iodinated contrast agents led to a statistically significant increase in DNA doublea??strand breaks after in vitro irradiation. This effect increased statistically significant with rising radiation dose and appeared independent of the contrast agent used (iopromid, iodixanol, iomeprol, iopamidol). A statistically significant difference in DNA damage between the different tested contrast agents was not found. Therefore, the increase in DNA doublea??strand breaks depends solely on the amount of iodine applied. For evaluation of clinical consequences, our findings could be tested in further animal studies. Copyright ?? 2014 John Wiley & Sons, Ltd.