Neurotropin? alleviates hippocampal neuron damage through a HIF‐1α/MAPK pathway

摘要

Summary AimsThe main purpose was to verify the potent capacity of Neurotropin? against neuronal damage in hippocampus and to explore its underlying mechanisms. MethodsHT22 cells were treated with 40?μmol/L Aβ25-35 in the presence of various concentrations of Neurotropin? or in its absence. The cell viability was assessed with a CCK-8 assay, and flow cytometry was used to measure cell apoptosis, intracellular ROS levels, and mitochondrial membrane potential. Aβ plaques were examined by Bielschowsky silver staining, and the activities of antioxidants were detected in hippocampus of APP/PS1 mice after Neurotropin? treatment. The expression of proteins, including HIF-1α, Bcl-2, Bax, and MAPKs signaling molecules was evaluated by Western blot. ResultsNeurotropin? significantly reversed the cell injury induced by Aβ25-35 through increasing cell viability and mitochondrial membrane potential, decreasing intracellular ROS and cell apoptosis of HT22 cells ( P ConclusionsNeurotropin? exhibited a potent neuroprotective effect on inhibiting Aβ-induced oxidative damage and alleviating Aβ deposition in hippocampus via modulation of HIF-1α/MAPK signaling pathway.