Protective Role of ( iR/isubiS/i/sub )‐glucoraphanin Bioactivated with Myrosinase in an Experimental Model of Multiple Sclerosis

摘要

Summary Aim The discovery of new natural compounds with pharmacological properties is a field of interest widely growing. Recent literature shows that B rassica vegetables ( C ruciferae) possess therapeutic effects particularly ascribed due to their content in glucosinolates, which upon myrosinase hydrolysis release the corresponding isothiocyanates. This study examines the potential neuroprotective and immunomodulatory effects of ( R S )‐glucoraphanin from T uscan black kale ( B rassica oleracea L. var. acephala sabellica) bioactivated with myrosinase (bioactive R S ‐ GRA ) (10 mg/kg/day intraperitoneally), in an experimental autoimmune encephalomyelitis ( EAE ), a model of multiple sclerosis. Methods EAE was induced by immunization with myelin oligodendroglial glycoprotein peptide ( MOG 35–55) in mice. After immunization, mice were observed daily for signs of EAE and weight loss. Clinical score was evaluated using a standardized scoring system. Results By Western blot analysis of spinal cord tissues, we have demonstrated that treatment with bioactive R S ‐ GRA significantly decreased nuclear factor ( NF )‐kB translocation, pro‐inflammatory cytokine production such as interleukin‐1β (IL‐1β), and apoptosis (Bax and caspase 3 expression). Conclusion Our results clearly demonstrate that bioactive R S ‐ GRA treatment may represent a useful therapeutic perspective in the treatment of this disease.