Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood

摘要

Background . We have shown that approximately 30% of human peripheral blood B-cells express CD25. B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity. The aim of the present study was to characterize the CD25-expressing subset of B cells in human cord blood. Material and Methods . Mononuclear cell fraction from human cord blood ( n = 34) and peripheral adult blood ( n = 22) was sorted into CD20~(+)CD25~(+)and CD20~(+)CD25~(?)B-cell populations. Phenotype and function of these B-cell populations were compared using flow cytometry, proliferation, cytokine production, and immunoglobulin secretion. Results . CD25-expressing B cells are a limited population of cord blood mononuclear cells representing 5% of the CD20~(+)B cells. They are characterised by high expression of CD5 in cord blood and CD27 in adult blood. CD25-expressing B cells express a functional IL-2 receptor and high levels of CC-chemokine receptors and spontaneously produce antibodies of IgG and IgM subclass. Conclusions . CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation, possibly ready to migrate and home into the peripheral tissue for further differentiation/action.