The?Coprinopsis cinerea?Tup1 homologue Cag1 is required for gill formation during fruiting body morphogenesis

摘要

The pileus (cap) of the fruiting body in homobasidiomycete fungi bears the hymenium, a layer of cells that includes the basidia where nuclear fusion, meiosis and sporulation occur.?Coprinopsis cinerea?is a model system for studying fruiting body development. The hymenium of?C. cinerea?forms at the surface of the gills in the pileus. In a previous study, we identified a mutation called?cap-growthless1-1?(cag1-1) that blocks gill formation, which yields primordia that never mature. In this study, we found that the?cag1?gene encodes a homologue of?Saccharomyces cerevisiae?Tup1. The?C. cinerea?genome contains another Tup1 homologue gene called?Cc.tupA. Reciprocal tagging of Cag1 and Cc.TupA with green and red fluorescent proteins revealed that the relative ratios of the amounts of the two Tup1 paralogues varied among tissues. Compared with Cc.TupA, Cag1 was preferentially expressed in the gill trama tissue cells, suggesting that the function of Cag1 is required for gill trama tissue differentiation and maintenance. Yeast two-hybrid analysis and co-localisation of Cag1 and Cc.TupA suggested that Cag1 interacts with Cc.TupA in the nuclei of certain cells.