Electroanalysis of cardioselective beta-adrenoreceptor blocking agent acebutolol by disposable graphite pencil electrodes with detailed redox mechanism

摘要

A simple economic graphite pencil electrode (GPE) was used for analysis of cardioselective, hydrophilic-adrenoreceptor blocking agent, acebutolol (ACBT) using the cyclic voltammetric, linear sweep voltammetric, differential pulse voltammetric (DPV), and square-wave voltammetric (SWV) techniques. The dependence of the current on pH, concentration, and scan rate was investigated to optimize the experimental condition for determination of ACBT. The electrochemical behavior of the ACBT at GPE was a diffusion-controlled process. A probable electro-redox mechanism was proposed. Under the optimal conditions, the anodic peak current was linearly proportional to the concentration of ACBT in the range from 1.00 to 15.0?μM with a limit of detection 1.26?×?10~(?8)?M for DPV and 1.28?×?10~(?8)?M for the SWV. This method was applied for quantitative determination of the ACBT levels in urine as real samples. The obtained recovery ranges for ACBT in urine were from 95.4 to101% as found by the standard addition technique. Further interference study was also carried with some common interfering substances.