6-Benzylamino purine and indol butyric acid on the in vitro multiplication of blackberry (Rubus idaeus), cv. Tupy

摘要

This work aimed to evaluate the effect of different BAP levels (0; 2; 4; 6; 8 and 10mM) and IBA (0; 0.5 and 1mM) on the in vitro multiplication of blackberry, cv. Tupy. The MS nutrient medium was supplemented with myo–inositol (100mg.-1), sucrose (30g.-1) and agar (6g.-1). The cultures were inoculated and then kept at a 16 hour-photoperiod, temperature of 25 ± 2?oC and 25?μmoles.m-2.s-1 radiation. The number of buds and shoots was evaluated weekly for one month. In the final evaluation, it was also taken into consideration the plantlet height and the rate of multiplication. In the absence of IBA, it was observed a higher number of buds, whereas the presence of BAP in the medium, promoted the highest number of buds at 2mM, which was achieved in the third week of cultivation. IBA at 0 and 1m M promoted a linear decrease in plantlet height when associated with increase in BAP concentrations. It was also observed that an increase in BAP levels for all IBA levels reduced the plantlet height. IBA at 1m M associated with BAP influenced negatively the rate of multiplication at 5.9mM BAP. BAP with no IBA addition promoted an increase in the rate of multiplication up to 5.1mM. IBA at 0.5mM, associated with all levels of BAP, did not influence significantly the rate of multiplication.