Towards the micropropagation of Euphorbia cyathophora Murray: a wild plant species with medicinal and ornamental potential

摘要

Euphorbia cyathophora Murray is a plant species with medicinal and ornamental potential but whose in vitro propagation is unknown. Two experiments were performed to know 1) the effect of light (WL) at 25?±2 ?°C and 20 μmol m-2 s-1 luminous intensity, or darkness at 20?±2 ?°C, for the first 20 days and subsequent placement in light (DKL) as previously described, and 2) the best combination of plant growth regulators: benzyl amino purine (BAP), kynetin (KIN), 2-isopentenyl adenine (2iP) and thidiazuron (TDZ) in combination with naphthaleneacetic acid (NAA) and the effect of both light conditions. The DKL treatment did not promote the growth of leaves, cotyledons, or plantlet height; in contrast, WL produced a more developed root system with early appearance of tertiary roots. In regards to the interaction of plant regulators and light effect, only the treatment with 1 mg BAP L-1 and 0.1 mg NAA L-1 generated shoots in both light conditions (DKL and WL). Darkness (DKL) generated more indirect greatest morphogenic responses (callus formation).