Determination of the Marker Diarylheptanoid Phytoestrogens in Curcuma comosa Rhizomes and Selected Herbal Medicinal Products by HPLC-DAD

摘要

A method for quantification of diarylheptanoids in Curcuma comosa rhizomes and selected pharmaceutical preparations was established by using HPLC-diode array detector (DAD). The chromatographic separation of three diarylheptanoids [(3 S )-1-(3,4-dihydroxy-phenyl)-7-phenyl-(6 E )-6-hepten-3-ol ( 1 ), (3 R )-1,7-diphenyl-(4 E ,6 E )-4,6-heptadien-3-ol ( 2 ), and (3 S )-1,7-diphenyl-(6 E )-6-hepten-3-ol ( 3 )] was performed on a Luna C₁₈ analytical column using gradient elution with 0.5% acetic acid in water and acetonitrile with a flow rate of 1?mL/min and a column temperature of 35°C. The calibration curves for the analytes showed good linearity ( R 2