Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system FREE

摘要

Technologies to achieve specific and precise genome editing, such as knock-in and knock-out, are critical for deciphering the functions of a gene and for understanding fundamental biological processes. Compared with the zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), which have been used for genome editing1, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system has emerged as a new powerful tool for genome modifications. It has recently been adopted for genome editing in human cell lines2,3,4, mouse5, zebrafish6, C. elegans7,8,9,10,11,12, and plants13.