LABORATORY DIAGNOSIS OPTIMIZING TESTS IN AMERICAN FULLBROAD

摘要

We examined 24 honey samples with sapling, and 4 of them(16,67%) contained Paenibacillus larvae. Bacteriological exam allowed us to isolate P. larvae after 3-5 days of inoculation period at 37C. On solid medium (glucose agar with 1 yolk of an egg 1/5 and BHI agar with 0,2 mg/l thiamine). In case of lichid growth, we isolated P. larvae after two days.The study of biochemical properties of P. larvae strains isolated from the four samples on API 20E galery confirmed that this belong to Paenibacillus larvae species subsp. larvae.Serological exam, trought VITA AFB Diagnostic Kit established the quick diagnostic (aproximatly 10 minutes), at the four samples witch were positive throught the classical bacteriological techniques, with new perspectives in optimising the diagnostics in American foulbrood.