New Perspectives on Asphodeline lutea from Bulgaria and Turkey: Anti-mutagenic, Anti-microbial and Anti-methicillin Resistant Staphylococcus aureus (MRSA) Activity

摘要

Aims: In this study, methanol extracts of Asphodeline lutea roots from Bulgaria (ALB) and Turkish (ALT) origin were evaluated for their anti-microbial, anti-MRSA properties and they were also screened for the potential of mutagenic and anti-mutagenic activities. Methodology: The broth micro dilution method was performed for the anti-microbial activities. For mutagenicity and anti-mutagenicity screening of the extracts, plate incorporation method of Ames test was employed. Results: Sarcina lutea was the most sensitive bacterium against ALB and ALT extracts at doses of 1.56 and 0.78 mg/ml, respectively. Both extracts exhibited similar activity against methicillin sensitive Staphylococcus aureus (MSSA) and Salmonella enteritidis with MIC value 6.25 mg/ml. Based on the results obtained from Ames test, no mutagenic activity was found for frame shift mutation (TA98) and base pair substitution (TA100) in all concentrations of A. lutea . Strong anti-mutagenic properties with rates of 77% and 75% were observed at the highest concentrations of both extracts against 2-aminofluorene-induced mutagenicity on TA 98 with the presence of metabolic activator S9 system. Conclusion: As a result, the extracts revealed significant anti-MRSA activity with MIC values 6.25 mg/ml against MRSA strains isolated from infections and manifested strong anti-mutagenic activity against known mutagens; it may be used in drug formulations against MRSA infections and may be used as a natural anti-mutagenic agent in the pharmacology and food industries.