Effect of met-enkephalin on chromosomal aberrations in the lymphocytes of the peripheral blood of patients with multiple sclerosis

摘要

Endogenious opiod met-enkephalin throughout previous research manifested cytoprotective and anti-in. ammatory e. ects. Previous research suggests that met-enkephalin has cytogenetic e. ects. Reducement in the frequency of structural chromosome aberrations as well as a suppres-sive e. ect on lymphocyte cell cycle is found. It also reduces apoptosis in the blood samples of the patients with immune-mediated diseases. Met-enkephalin exerts immunomodulatory properties and induces stabilization of the clinical condition in patients with multiple Sclerosis (MS). Th e goal of the present research was to evaluate met-enkephalin in vitro e. ects on the number and type of chromosome aberrations in the peripheral blood lymphocytes of patients with MS. Our research detected disappearance of ring chromosomes and chromosome frag-mentations in the cultures of the peripheral blood lymphocytes treated with met-enkephalin (... μg/mL). However, this research did not de-tect any signifi cant e. ects of met-enkephalin on the reduction of structural chromosome aberrations and disappearance of dicentric chromo-somes. Chromosomes with the greatest percent of inclusion in chromosome aberrations were noted as: chromosome ., chromosome . and chromosome .. Additionally, we confi rmed chromosome .. as the most frequently included in translocations. Furthermore, met-enkephalin e. ects on the increase of the numerical aberrations in both concentrations applied were detected. Th ose fi ndings should be interpreted cau-tiously and more research in this fi eld should be conducted