Utility of matK Gene as DNA Barcode to Assess Evolutionary Relationship of Important Tropical Forest Tree genus Mangifera (Anacardiaceae) in Indonesia and Thailand

摘要

MaturaseK ( mat K) gene of chloroplast DNA has been served as appropriate candidate to be a DNA barcode in angiosperms. Using this DNA marker, 19 species of genus Mangifera , one of ecologically important crop, collected from Indonesia and Thailand were analyzed . Phylogenetic analysis using parsimony method revealed that the gene could clasify Mangifera into three major groups , namely group I, II, and III . Moreover, the mat K barcode can identify Mangifera species that is originated from Thailand. Although this classification system is different with the previous system, it can provide a new information about Mangifera taxonomy. Results further exhibited that DNA sequences of the mat K of two Mangifera species ( M. laurina dan M. macrocarpa ) are different between Indonesia and Thailand specimens. ? Keywords — DNA barcode, Mangifera , mat K gene, p arsimony, p hylogenetic a nalysis