FGF2 crosstalk with Wnt signaling in mediating the anabolic action of PTH on bone formation

摘要

The mechanisms of the anabolic effect of parathyroid hormone (PTH) in bone are not fully defined. The bone anabolic effects of PTH require fibroblast growth factor 2 (FGF2) as well as Wnt signaling and FGF2 modulates Wnt signaling in osteoblasts. In vivo PTH administration differentially modulated Wnt signaling in bones of wild type (WT) and in mice that Fgf2 was knocked out (Fgf2KO). PTH increased Wnt10b mRNA and protein in WT but not in KO mice. Wnt antagonist SOST mRNA and protein was significantly higher in KO group. However, PTH decreased Sost mRNA significantly in WT as well as inFgf2KOmice, but to a lesser extent inFgf2KO. Dickhopf 2 (DKK2) is critical for osteoblast mineralization. PTH increasedDkk2mRNA in WT mice but the response was impaired inFgf2KOmice. PTH significantly increased Lrp5 mRNA and phosphorylation of Lrp6 in WT but the increase was markedly attenuated inFgf2KOmice. PTH increased β-catenin expression and Wnt/β-catenin transcriptional activity significantly in WT but not inFgf2KOmice. These data suggest that the impaired bone anabolic response to PTH inFgf2KOmice is partially mediated by attenuated Wnt signaling.