Elimination kinetics of eugenol in grass carp in a simulated transportation setting

摘要

Fish are vulnerable to stress from over-crowding during transportation and eugenol is the most common sedative used to minimize fish injury. The ADI value of 2.5?mg/kg is recommended by the Joint FAO/WHO Expert Committee on Food Additives. The aim of this work was to study the elimination kinetics of eugenol following exposure of grass carp to a eugenol bath in a simulated transportation setting. Grass carp, Ctenopharyngodon idella (120 fish) were exposed for 24?h to a 10?mg/L eugenol bath. Sampling was performed during a 96?h period after the 24?h bath. Eight fish were sampled at each time point and muscle, plasma and liver concentrations of the drug were determined by ultra-performance liquid chromatography-tandem mass spectrometry. The concentration-time data of eugenol in each tissue were analyzed using non-compartmental methods. The peak concentrations (Cmax) in plasma, muscle and liver were 7.68, 5.30 and 24.63?mg/kg and the elimination half-lives (t1/2β) were 19.79, 10.27 and 55.28?h, respectively. The clearance (CL) values were 0.10, 0.44 and 0.04?L/h/kg and the areas under the concentration-time curve (AUC0-96h) were 91.54, 22.44, and 214.12?mg·h/L in plasma, muscle and liver, respectively. After a eugenol exposure bath, drug concentrations in muscle tissue of grass carp were below 1?mg/kg at 8?h and 0.1?mg/kg at 24?h. The drug concentrations in muscle tissue at 8?h were lower than the recommended ADI value.