Biological screening and bioguided fractionation of extracts of the brown alga Cystoseira tamariscifolia the Moroccan coast: Antibacterial activity and cytotoxicity test

摘要

In this study, we screened for the fractionation of extracts of the brown algae Cystoseira tamariscifolia the Moroccan coast. Selecting this alga was based on the results of biological activity on the extracts obtained after Soxhlet extractionwith solvents of increasing polarity such as hexane, ether, chloroformand water.We followed two biological activities: (a) the antibacterial activity in the agar diffusion test against Klebsiella pneumoniae and Enterobacter cloacae, and (b) cytotoxicity assay to brine shrimp (Artemia salina). After these preliminary tests, we selected the ether extract of this alga for fractionation, where we collected 11 fractions per gradient of petroleumether / ethyl acetate. The collected fractions from the ether extract also tested for antibacterial activity and cytotoxicity test. The overall results showthat the fractions fromether extract of Cystoseira tamariscifolia exhibit remarkable biological activity.