High yield purification of Helicobacter pylori neutrophil-activating protein overexpressed in Escherichia coli

Yu-Chi Yang; Ting-Yu Kuo; Zhi-Wei Hong; Han-Wen Chang; Chung-Chu Chen; Te-Lung Tsai; Hua-Wen Fu

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High yield purification of Helicobacter pylori neutrophil-activating protein overexpressed in Escherichia coli

机译：高产量纯化在大肠杆菌中过表达的幽门螺杆菌嗜中性粒细胞活化蛋白

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Background Helicobacter pylori neutrophil-activating protein (HP-NAP) is involved in H. pylori -induced gastric inflammation. Due to its immunogenic and immunomodulatory properties, HP-NAP has been used for developing vaccines against H. pylori infection and new drugs for cancer therapy.

机译：背景幽门螺杆菌嗜中性粒细胞活化蛋白（HP-NAP）参与幽门螺杆菌诱导的胃炎症。由于其免疫原性和免疫调节特性，HP-NAP已被用于开发抗幽门螺杆菌感染的疫苗和用于癌症治疗的新药。

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《BMC Biotechnology》 |2015年第1期|共页
作者
Yu-Chi Yang; Ting-Yu Kuo; Zhi-Wei Hong; Han-Wen Chang; Chung-Chu Chen; Te-Lung Tsai; Hua-Wen Fu;
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中图分类生物工程学（生物技术）;
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1. The neutrophil-activating Dps protein of Helicobacter pylori, HP-NAP, adopts a mechanism different from Escherichia coli Dps to bind and condense DNA [J] . Ceci P, Mangiarotti L, Rivetti C, Nucleic Acids Research . 2007,第7期

机译：幽门螺杆菌的嗜中性粒细胞激活Dps蛋白HP-NAP采用不同于大肠杆菌Dps的机制来结合和浓缩DNA
2. The neutrophil-activating Dps protein of Helicobacter pylori, HP-NAP, adopts a mechanism different from Escherichia coli Dps to bind and condense DNA [J] . Claudio Rivetti, Emilia Chiancone, Laura Mangiarotti, Nucleic acids research . 2007,第7期

机译：幽门螺杆菌的嗜中性粒细胞激活Dps蛋白HP-NAP采用不同于大肠杆菌Dps的机制来结合和浓缩DNA
3. Studies on the maximization of recombinant Helicobacter pylori neutrophil-activating protein production in Escherichia coli: application of Taguchi robust design and response surface methodology for process optimization [J] . Fontani S, Niccolai A, Kapat A, World Journal of Microbiology & Biotechnology . 2003,第7期

机译：大肠杆菌中重组幽门螺杆菌嗜中性粒细胞激活蛋白产量最大化的研究：Taguchi稳健设计和响应面方法在工艺优化中的应用
4. B-cell epitope mapping of Helicobacter pylori neutrophil-activating protein [C] . Chibing Gong, Yan Li, Ming Li, Frontiers in the Convergence of Bioscience and Information Technologies . 2007

机译：幽门螺杆菌中性粒细胞活性蛋白的B细胞表位映射
5. The purification of proteins having properties of an N-methyl-D-aspartate (NMDA) receptor from brain synaptic membranes and characterization of a glycine-binding protein expressed in Escherichia coli. [D] . Babcock, Kent Kincaid. 1994

机译：从脑突触膜纯化具有N-甲基-D-天冬氨酸（NMDA）受体特性的蛋白质，并表征在大肠杆菌中表达的甘氨酸结合蛋白。
6. High yield purification of Helicobacter pylori neutrophil-activating protein overexpressed in Escherichia coli [O] . Yu-Chi Yang, Ting-Yu Kuo, Zhi-Wei Hong, 2015

机译：高产量纯化在大肠杆菌中过表达的幽门螺杆菌嗜中性粒细胞激活蛋白
7. The neutrophil-activating Dps protein of Helicobacter pylori, HP-NAP, adopts a mechanism different from Escherichia coli Dps to bind and condense DNA [O] . Ceci, Pierpaolo, Mangiarotti, Laura, Rivetti, Claudio, 2007

机译：幽门螺杆菌的嗜中性粒细胞激活Dps蛋白HP-NAP采用不同于大肠杆菌Dps的机制来结合和浓缩DNA

High yield purification of Helicobacter pylori neutrophil-activating protein overexpressed in Escherichia coli

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