The Effect of Myricetin Flavonoid on the Expression of Fyn Gene in Melanoma Cells (A375)

摘要

Background: Malignant melanoma as one of the most common cancers is currently spreading worldwide. Regarding after-effectof advanced treatments, using natural products has attracted much attention. Flavonoids, polyphenol compounds rich in diet, arebeing considered for their therapeutic preventive features. Fyn gene, a member of the protein tyrosine kinase oncogene family, hasbecome an important target for therapy goals.Objectives: The aim of this study was to assess Fyn gene expression after treatment of melanoma cells with myricetin.Methods: In this study, the melanoma cells were treated with different concentrations of myricetin (0 to 100 M) and their viabilitywas determined by the methylthiazolyl diphenyl-tetrazolium bromide (MTT) assay, also the expression of Fyn gene in treatedcells with selected concentrations of myricetin (0, 20, 40, 50, and 60 M) was detected by real time quantitative polymerase chainreaction (qPCR).Results: The current investigation showed that treatment of A375 melanoma cells with the dietary flavonoid myricetin (3, 5, 7-trihydroxy-2-(3, 4, 5,-trihydroxy phenyl)-4- chromenone), resulted in decreased cell viability and increased expression of Fyn gene.The MTT assay analysis of exposed cells with different concentrations of myricetin showed that up to 25 Mof myricetin had nocytotoxicity effect on A375 cells, also with increasing of myricetin concentration, the repression of cell proliferation developed aswell.Conclusions: Real time qPCR analysis of Fyn expression in exposed cells with various concentration of myricetin leads to overexpressionof this gene, dose dependently. Through this research, it was determined that myricetin with its anti-proliferative potentialcould suppress the development of cancer cells. On the other hand, since Fyn kinase could be involved in tumorigenesis of somecancer cells, it could be concluded that myricetin could effect the carcinogenicity of Fyn function in melanoma cells.