Effect of intra-abdominal cisplatin on post-laparotomy adhesion bands in rats

摘要

Background: Intra-abdominal adhesion is one of the major problem of postoperative surgery. It causes abdominal, and gynaecological morbidity. Postoperative intraperitoneal chemotherapy (IPCT) has been proved to be effective in patients with advanced cancer after cytoreductive surgery compared to systemic chemotherapy. In recent years there are several studies have been conducted to evaluate the role of IPCT on cancer treatment wound healing, and adhesion formation.Objectives: The aim of this experimental study was to evaluate the effect of intra-abdominal cisplatin on postoperative adhesion formation during 2nd and 4th weeks after surgery.Method: For conducting this experimental study, 40 male Wistar rats were studied and divided into two groups of twenty animals each. The abdomen was prepared for laparotomy. They underwent a laparotomy through a 3 cm midline incision. Then five abrasions as 3-4 cm were made on cecum the peritoneal wall. Control and treatment groups were received saline 0.9% (2 ml) and cisplatin (2 mg/kg) respectively during laparotomy intra-abdominally. Adhesion grade score was observed 2nd and 4th weeks after laparotomy.Results: As the results, 10% of animals in the cisplatin groups have zero adhesion score compared to control group (11%) 4th week after surgery that this difference was not significant (P=0.964). The control group with score 3 (44.4%), and cisplatin group with score 1 (55.6%) had most adhesion in the 2nd week. Most adhesion were happened with score of 1 and 3 for both control (33.3%) and cisplatin groups (40%) respectively, 4th week after surgery (P=0.964). Moreover, there was no significant difference between two groups as the adhesion score (0-1) and (2-3) in the 2nd and 4th weeks after surgery (P=0.75).Conclusion: Administration of intra-abdominal cisplatin in rats during laparotomy could induce adhesion band similar to control group. It seems that notice to chemotherapeutic agents, range of doses, number of pulse therapy, time of administration after operation, improving their formulation, and solvents application are necessary.