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Use of Rapd Fingerprinting for Study and Conservation of Fish Populations

机译:Rapd指纹技术在鱼类种群研究和保护中的应用

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This paper analysed a total of 76 fish individuals collected at four different localities in Republic of Macedonia. The following were encompassed: River Strumica, River Vardar and two natural lakes-Ohrid Lake and Prespa Lake. The samples affiliated with the four previously mentioned barbel species on the territory of Republic of Macedonia: Barbus cyclolepis Kar., Barbus prespensis Kar., Barbus macedonicus Kar. and Barbus peloponnesius Val. The molecular RAPD method was used for investigation of the population structure of the Barbus genus. A total of ten oligonucleotide primers was used to obtain various RAPD profiles in different species. The established genetic markers allow an opportunity for exact identification of the species and populations of barbel and for detection of interspecies hybrids.
机译:本文分析了在马其顿共和国四个不同地区收集的总共76条鱼类个体。其中包括:斯特鲁米察河,瓦尔达尔河和两个自然湖-奥赫里德湖和普雷斯帕湖。这些样品与马其顿共和国境内先前提到的四种口须物种有关:Barbus cyclolepis Kar。,Barbus prespensis Kar。,Barbus macedonicus Kar。和Barbus peloponnesius Val。分子RAPD方法用于调查Barbus属的种群结构。总共使用了十个寡核苷酸引物来获得不同物种的各种RAPD图谱。既定的遗传标记为准确鉴定杠铃的种类和种群以及检测种间杂种提供了机会。

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