The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process

摘要

Background To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells. Methods Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000?μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α?=?0.05). Results Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250?μg/mL which was statistically significant ( p ?0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100?μg/mL, respectively with no significant difference ( p >?0.05) compared to control, while concentrations above these conferred inhibitory effects. Conclusion Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.