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Heterotrophic cultivation of Nannochloropsis salina for enhancing biomass and lipid production

机译:异养拟南芥盐酸盐的培养,以提高生物量和脂质的产生

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Response surface methodology (RSM) was used to enhance the biomass and lipid content in Nannochloropsis salina due to its economic importance. Preliminary screening results revealed that the heterotrophically cultivated N. salina with various carbon and nitrogen sources yielded higher biomass (0.91+/-0.0035g/L) and lipid content (37.1+/-0.49mg/L) than that of the photoautotrophical cultivation (0.21+/-0.009g/L and 22.16+/-0.27mg/L). Significant sources that greatly influenced on biomass and lipid content of the alga were optimized through RSM. The medium consisting of glucose (7.959g/L), sodium acetate (1.46g/L), peptone (7.6g/L) and sodium thiosulphate (1.05g/L) was found to be the optimal concentration for heterotrophic cultivation by response optimizer. Confirmation experiment results for the RSM optimized concentration yielded the biomass of 1.85g/L and total lipid content of 48.6mg/L. In this study, we provide with a strategy for enhancing the biomass and lipid content in N. salina.
机译:由于其经济重要性,使用响应表面方法(RSM)来提高拟南芥盐沼中的生物量和脂质含量。初步筛选结果表明,与光合自养栽培相比,具有多种碳源和氮源的异养栽培盐生猪笼草的生物量(0.91 +/- 0.0035g / L)和脂质含量(37.1 +/- 0.49mg / L)更高。 0.21 +/- 0.009g / L和22.16 +/- 0.27mg / L)。通过RSM优化了对藻类生物量和脂质含量有重大影响的重要来源。响应优化器发现,葡萄糖(7.959g / L),乙酸钠(1.46g / L),蛋白one(7.6g / L)和硫代硫酸钠(1.05g / L)组成的培养基是异养栽培的最佳浓度。 。 RSM优化浓度的确证实验结果得出生物量为1.85g / L,总脂质含量为48.6mg / L。在这项研究中,我们提供了一种策略,以提高盐沼猪笼草的生物量和脂质含量。

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