Oridonin alters the expression profiles of MicroRNAs in BxPC-3 human pancreatic cancer cells

摘要

Background Oridonin, an ingredient used in traditional Chinese medicine, has been demonstrated to play an important role in antitumour effects, but the mechanism underlying its antitumour properties is still not clear. Methods To verify the anti-cancer effects of oridonin via a miRNA-dependent mechanism, comprehensive miRNA expression profiling of oridonin-treated BxPC-3 human pancreatic cancer cells was performed using a miRNA microarray assay based on Sanger miR-Base Release 20, followed by a validation using real-time PCR. MicroRNA target prediction and Gene Ontology and KEGG pathway analysis were performed to investigate possible pathways involved. Results The results showed that 105 miRNAs were significantly differentially expressed (signal reading >500, p?≤?0.01, |Log2-value| ≥1) in oridonin-treated BxPC-3 human pancreatic cancer cells. Conclusions Our data indicates that oridonin inhibits BxPC-3 cells probably through regulating the expression of miRNAs. Interruption of miRNA profiling may provide new therapeutic methods for the clinical treatment of pancreatic cancer.