Follow-Up of Implant Survival Comparing Ficoll and Bone Marrow Aspirate Concentrate Methods for Hard Tissue Regeneration with Mesenchymal Stem Cells in Humans

摘要

Objective: Clinical follow-up of implant survival in 11 patients comparing two different methods for mesenchymal stem cell (MSC) isolation (Ficoll and bone marrow aspirate concentrate [BMAC]) applied in maxillary sinus augmentation. Methods: Mononuclear cells, including MSCs, were concentrated with either Ficoll (control group, n =6 sinus) or BMAC (test group, n =12 sinus) and transplanted in combination with bovine bone mineral. A total of 50 implants were placed in a second surgical intervention (17 Ficoll/33 BMAC) and loaded after 4 months. Overall implant survival was assessed with a Kaplan-Meier model using package survival under R. Results: Implant survival of the Ficoll group was 100% compared with the BMAC group, which had 93.4% survival (95% confidence interval, 0.849–1). The difference between the groups was not significant ( p =0.381). Conclusion: The BMAC system is an effective and suitable “chair-side” method for clinical application in hard tissue regeneration.