POLYMORPHISM OF VASCULAR ENDOTHELIAL GROWTH FACTOR REGIO PROMOTER C(-634)G AS A RISK FACTOR OF BALINESE TYPE-2 DIABETIC RETINOPATHY

摘要

Background: Diabetic retinopathy (DR) is one of the complications on diabetic mellitus (DM) patientas a micro vascular retina disorder which caused by a long term of hyperglycemia. This is one of theblindness causes in the world. This research aims to find out Polymorphism of VEGFC(-634)G geneas a risk factor of DR on the Balinese with DM type-2 (DMT2). Method: This study is applying twodesigns, analytical cross sectional and case control. The case is DMT2 patient with DR(+), DMT2without DR as control. The sequencing technique was performed to evaluate polymorphism andplasma VEGF levels were determined by ELISA. Results: Cross sectional study (hospital based) cameout with quite high number of DR, 57 people of 97 study samples. This study shows the existence ofgenetic variation on Gen VEGF C(-634)G, with most often genotype of CG (51.5%). PolymorphismC(-634)G as the risk factor of DR (OR=1.815 CI-95%= 1.077-3.057, p=0.025), and high level ofVEGF were also significant (QR=3.75 , CI-95% 1.34-10.20, p=0.008). VEGF level with genotype ofCG, CC and GG, not found any difference (p=0.245). Logistic regression shows that the mostinfluential variable as the risk factor of DR is VEGF level (p= 0.007), polymorphism gen VEGF C(-634)G (p=0.022) and systolic blood pressure (p=0.023). Conclusions: Polymorphism of VEGF C(-634)G gene and high level of VEGF as the risk factor of DR, and can be used as a reference inhandling early stage of DR patient to prevent blindness.