Design and Manufacturing of Electromechanical Chip for Rapid Measurement of IgG1 Antibody in Cell-Culture Supernatant

摘要

In this study, we have used arrays of piezoresistive nanomechanical membrane resonators (NMRs) to detect IgG1antibody. The NMR consists of a membrane suspended by four piezoresistive sensing components. Unlike conventionalcantilever mass sensors, our sensors retain a uniform mass sensitivity over the membrane surface. According to theexperiments, it was revealed that NMRs have mass sensitivities in the order of 25 Hz/pg in air and 1.8 Hz/pg in liquid.This matter allows them to detect IgG1 over a wide range of concentrations from 100 pg/mL to 10 μg/mL in a backgroundof human serum albumin (HSA) at 1 mg/ml. This indicates the fact that the self-sensing NMR approach is beneficial fordetecting disease markers. Moreover, the performance of the NMR was compared with other detection methods, andthe results indicated a better performance for NMR.