Application of canonical discriminant analysis in differentiation of natural populations of Pinus nigra in Serbia based on terpene composition

摘要

?arac, Z., Bojovi?, S., Nikoli?, B., Zlatkovi?, B., Marin, P.: Application of canonical discriminant analysis in differentiation of natural populations of Pinus nigra in Serbia based on terpene composition. Biologica Nyssana, 5 (1), September 2014: 11-15. The canonical discriminant analysis (CDA) was performed in order to check the hypothesis of chemical separation infraspecific taxa of Pinus nigra J.F. Arnold (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica) in Serbia based on variability of the needle terpenes. The CDA, which maximizes variations between a priori groups, showed division of seven native P. nigra populations into three groups, which belong to three taxonomically recognized taxa (ssp. nigra, ssp. pallasiana, and var. banatica). The most important characters in discrimination were (E)-caryophyllene, α-humulene, terpinolene, germacrene D, α-pinene, and myrcene. The individuals of pallasiana group were poorer in (E)-caryophyllene and terpinolene and richer in α-humulene contrary to the individuals of nigra group. The individuals of banatica group had the highest content of α-pinene and myrcene. The obtained results were compared with recently published data on chemodiversity of P. nigra infraspecific taxa on the territory of Serbia.