Study of Molecular Mechanisms of PDLIM4/RIL in Promotion of the Development of Breast Cancer

摘要

PDLIM4 (RIL) gene is often subjected to epigenetic suppression in malignant tumors of various organs. At the same time, reasons of this suppression are not fully understood, and the only effect of the proposed mechanism of PDLIM4 impact on malignant transformation of epithelial cells is partially refuted by new experimental data. In order to determine a range of targets, which can be affected by changes in the level of PDLIM4 expression, a model system based on the breast cancer cell line MDA-MB-231 was created and characteristics were given to the model. The model had artificially suppressed PDLIM4 expression by introduced shRNA expression promoter. Comparative transcriptome sequencing before and after the suppression of PDLIM4 revealed a number of genes whose expression levels correlated with a change in PDLIM4 expression. Most of these genes related to the MAPK signal pathway. Based on the data obtained, the most likely candidate genes were offered, which may play the role of the end effectors that stimulate malignant transformation after PDLIM4 suppression. *Corresponding author: Frolova EI, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 16/10 Ulitsa Miklukho- Maklaya, GSP-7, Moscow 117997, Russian Federation; E-mail: eifr@ibch.ru Received: Jan 11, 2016; Accepted: Mar 14, 2016; Published: Apr 13, 2016 Citation: Kravchenko DS, Lezhnin YN, Kravchenko JE, Chumakov SP, Frolova EI (2016) Study of Molecular Mechanisms of PDLIM4/RIL in Promotion of the Development of Breast Cancer. Biol Med (Aligarh) 8(2): BM-175-16, 6 pages. Copyright: . 2016 Kravchenko et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.