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机译:优化的新型GLP-1类似物rolGLP-1在大肠杆菌BL21(DE3)中的表达,纯化及对2型糖尿病小鼠的良好促糖调节作用
Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, P.R. China;
Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, P.R. China;
Food Science & Human Nutrition Dept. University of Florida, Gainesville, FL, 32611, USA;
Tianjin Agricultural University, Department of Basic Science Tianjin 300384, P.R. China;
Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, P.R. China;
Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, P.R. China;
Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, P.R. China;
Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, P.R. China;
Food Science & Human Nutrition Dept. University of Florida, Gainesville, FL, 32611, USA;
Key Laboratory for Bioactive Materials of the Ministry of Education, Institute of Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, P.R. China;
Diabetes; expression; GLP-1; Oral glucose tolerance; Purification; Western Blotting;
机译:口服含有在链脲佐菌素诱导的糖尿病大鼠的大肠杆菌BL21(DE3)中产生的八种GLP-1类似物的融合蛋白
机译:重组蛋白在大肠杆菌中的毒性:在BL21(DE3),C41(DE3)和C43(DE3)中过表达的比较
机译:通过选择性引入同义稀有密码子来增强Codon优化异源酶的功能性表达
机译:与芽孢杆菌磷脂酶C的重组人体表皮生长因子(RHEGF)的共同表达
机译:使用两种不同的破裂方案:渗透压休克和细胞裂解,从大肠杆菌BL21中纯化周质结合的重组志贺毒素B蛋白。
机译:使用表面反应方法优化Mpt64合成基因在大肠杆菌BL21(DE3)中表达的培养条件
机译:表1:从大肠杆菌BL21(DE3)帘布层作为表达系统,从新构建的GSTM-T1脂肪酶纯化T1脂肪酶。