Phytoplankton Toxins in the Central Adriatic Sea

摘要

Toxin analysis of shellfish from the Central Adriatic Sea, for which no previous studies exist, have been carried out. Investigations, using the bio-assay and HPLC-directed analysis, led to identification of the toxins responsible for diarrheic shellfish poisoning (DSP). Okadaic acid (OA), a C_(38) polyether fatty acid derivative, and its 35-methyl derivative dinophysistoxin-1 (DTX-1) were identified as the principal toxin components. DSP toxins were detected in mussels sampled in the Kastela Bay. Results are presented along with the phytoplankton structure during the outbreak of DSP toxicity.%Prikazani su rezultati istraživanja sekundarnih metabolita dinoflagelata i njihove uloge u humanoj intoksikaciji, koja su po prvi put provedena u vodama srednjeg Jadrana. Toksičnost školjkaša ispitana je pomoću bioassay-a i visokotlačne tekućinske kromatografije (HPLC). Utvrđena je prisutnost toksina odgovornih za diarorično trovanje školjkama DSP (diarrheic shellfish poisoning). Okadaična kiselina (C_(38) polieter) i njen 35-metilni derivat, dinofizistoksin-1, identificirani su kao osnovni uzročnici toksičnosti.